Emma Raducanu withdrew from her ASB Classic second round match in tears after injuring her ankle just 11 days before the start of the Australian Open.
The 20-year-old Brit won the first set 6-0 against Slovakia Viktoria Kuzmova, but lost the second 7-5.
She received medical attention before the start of the third set, but signaled her retirement two points after her opening service game.
The former US Open champion had an injury-plagued 2022 season.
Having withdrawn from five games last year, Raducanu said he was no injuries and feeling “fine” after an exhibition game in December.
Started 2023 with victory over teenager Linda Fruhvirtova in their first match in Auckland in preparation for the first major tournament of the season in Melbourne.
Elsewhere, on Thursday, Coco Gauff defeated fellow American and 2020 Australian Open winner Sofia Kenin 6-4 6-4.
The 18-year-old number one seed will play in the quarterfinals against China’s Zhu Lin, who defeated seven-time Grand Slam singles champion Venus Williams 3-6, 6-2, 7-5 in a game interrupted by rain.
Zhu won the last four games of the match when he came back from 5-3 in the deciding set. Williams served for the match at 5-4, but the Chinese World No.84 broke and saved four break points as she held serve to advance 6-5 before breaking serve again.